OBJECTIVE :
To involve in a powerful team that achieves extraordinary results with complete job satisfaction. Also to be found in a mutually beneficial longterm relationship, in which I can share ideas, interact, discover and explore opportunities, a situation that helps me to consistently develop my skills.
TRAINING UNDERGONE :
Implant training in Department of Medicine Manufacturing section Pankajakasthuri, Thiruvananthapuram, Kerala, India.
SEMINAR AND WORKSHOP ATTENDED :
Participated in National level seminar onRenewable energy, Sponsored by DRDO;conducted by Department of Biotechnology, Udaya School of Engineering.
Participated in National Seminar onEnvironmental Conservation by Traditional and Modern Approaches;conducted by Department of Biotechnology, Udaya School of Engineering.
Participated in two days National seminar on Recent Advances in Biotechnology; conducted by Department of Biotechnology, Udaya School of Engineering.
PAPER PRESENTED :
Presented a paper on “Regulation of diesel oil in a polluted soil using Bacillus subtilis” at National Technical Symposium. Conducted by Department of Biotechnology, Sathyabama University, Chennai, India.
PROJECT PRESENTED :
Presented a project on “Purification of bovine serum albumin from Pichia pastoris fermented broth” at National level Technical Symposium conducted by Department Of Biomedical, Udaya School Of Engineering, Nagercoil, Tamilnadu, India.
TECHNICAL PROFICIENCY :
1. Basic Biochemistry Basic tools, Quantitative and Qualitative assay of Biomolecules Chromatography (Paper, Column, and TLC)
2. Immunology& Microbiology Basic immunology techniques, Basic Microbiology techniques. Separation and purification of lymphocytes.
3. Bioprocess Engineering and Bioseparation Enzyme kinetic analysis, Monod Kinetic Analysis, Sonication, Protein precipitation.
4. Molecular Biology and Genetic Engineering. Isolation of DNA from plant, Bacteria and Animal tissue. Competent cell preparation, Transformation, Electophoretic techniques (SDSPAGE and AGE).
5. Cancer Biology Antimutagenesis study, CBMN Assay, Basics in Cell Culturing.
AREAS OF INTEREST :
Cancer Biology, Molecular Biotechnology,Immunology,pharmaceutical biotechnology.
EDUCATIONAL QUALIFICATION :
EXAM PASSED INSTITUTION AND UNIVERSITY YEAR OF PASSING PERCENTAGE
B.Tech (Biotechnology) Udaya School of Engineering, Anna University, Thirunelveli March 2011 72%
Plus Two Govt : Model Boys H S S Attingal, Trivandrum. March 2006 70%
SSLC S S P B H S Kadakkavur, Trivandrum. March 2004 74%
GRADUATE COURSE :
B.Tech (Bachelor of Technology) in Biotechnology
Major Subjects Studied;
Cell Biology Microbiology
Biochemistry Molecular Biology
Genetic Engineering Chemical Engineering
Chemical Reaction Engineering Bioprocess Engineering
Protein Engineering Animal Biotechnology
Plant Biotechnology Mass Transfer Operations
Environmental Biotechnology Immunology
Bioseparations Bioinformatics
Biopharmaceutical technology
COMPUTER SKILLS :
Operating Systems
XP, Vista
General Applications MS Office
Keen user of the Internet.
STRENGTHS :
Achievement oriented with excellent people management skills and an ability to manage change with ease.
Proven strength in coordination.
Strong communication, interpersonal and learning skills matched with the ability to manage stress, time and people effectively.
ABSTRACT OF PROJECT :
PARTIAL PURIFICATION OF RECOMBINANT BOVINE SERUM ALBUMIN FROM Pichia pastoris FERMENTED BROATH.
Bovine serum albumin (BSA) is used as a diluent or a blocking agent in numerous applications including ELISAs (EnzymeLinked Immunosorbent Assay), blots and immune histochemistry.
Additionally, native BSA is routinely used to enhance the stability of proteins during or after their purification, or in biological assays and to prevent adhesion of the enzyme to reaction tubes and other vessels.
In recent years, there has been a mounting concern over the use of animal products in many pharmaceutical and biotech industry applications because of the threat of animal viruses and transmissible spongeform ensephelopathies (TSEs; the causative agent of mad cow disease) that may be present in bovinederived BSA. Current methods of preparation of BSA involve purification of the protein from bovine plasma and testing for the presence of these contaminating agents.. Therefore, there is currently no alternative largescale supply of BSA that is assured to be free of all animalderived infectious agents. So we want to optimize a method for BSA purification in small scale which is scalable. The source of the protein is from recombinant production.
Purification of recombinant BSA produced from fermentation involves extraction, primary, secondary purification. Different techniques applied in purification based on their molecular properties like isoelectric point, surface charge, molecular weight, hydrophobicity, affinity, sedimentation coefficient etc. optimization involves centrifugation, microfiltration, precipitation techniques, different chromatography, Tangential flow filtration techniques, and desalting methods . The pure form of BSA obtained after purification should be sterile and free of impurities when compared with known standard.
DECLARATION :
I hereby confirm that the information furnished above is correct to the best of my knowledge.
DATE : ___________
PLACE : ___________